Project Details Methods to evaluate the scavenging activity of antioxidants toward reactive oxygen and nitrogen species (ROS/RNS)

Project No.:
2013-015-1-500
Start Date:
01 September 2013
End Date:
Division Name:
Analytical Chemistry Division
Division No.:
500

Objective

To identify the quenching chemistry of biologically important reactive oxygen and nitrogen species (ROS/RNS, including free radicals); to show antioxidant action against reactive species through H-atom and electron transfer reactions, and to evaluate the ROS/RNS scavenging activity of antioxidants with existing analytical methods while emphasizing the underlying chemical principles and advantages/disadvantages of these methods. In addition to discussing various methodologies with respect to kinetics and thermodynamics, the project will focus on the applications and impact of existing assays on potentiating future research and innovations to evolve better methods enabling more comprehensive study of different aspects of antioxidants, and to provide a vocabulary of terms related to antioxidants and scavengers for ROS/RNS.

Description

Reactive oxygen/nitrogen species (ROS/RNS) generation is directly related to oxidative degradation in foods and biological systems. Therefore, the search for methods to determine the scavenging activity of reactive species is very important. Determination of the antioxidant/antiradical status in these systems could contribute to prevention and treatment of diseases related to aging such as cardiovascular and neurodegenerative diseases and cancer. In addition to organism defences, the intake of dietary antioxidants and influence of real contribution of foods to antioxidant status in biological systems must be evaluated [1]. The main methods comprise the scavenging activity measurement of hydroxyl radical (HO.), superoxide anion radical (O2.-), hydrogen peroxide (H2O2), hypochlorous acid (HOCl), peroxynitrite (ONOO), and peroxyl radical (ROO.). In spite of the diversity of methods, there is currently a great need to evaluate the scavenging activity of antioxidant compounds in vivo and in vitro. In addition, there are incorrect methods frequently used, such as non-selective UV measurement of H2O2 scavenging, producing negative errors due to incomplete reaction of peroxide with flavonoids in the absence of transition metal ion catalysts [2]. The Chair’s research collaborators have recently published a comprehensive review on “methods of measurement and evaluation of natural antioxidant capacity/activity” [3]. Complementary to this technical report, this project will provide chemical information about methods clarifying reaction mechanisms, thermodynamic scavenging efficiency and kinetic scavenging rates of antioxidants. Moreover, it will aid the identification of reactive species and quantification of scavenging extents of antioxidants through various assays, make the results comparable and more understandable, and bring a more rational basis to the evaluation of these assays. Modern nano-technological methods for the estimation of reactive species and their scavenging action will also be discussed.

References:

[1] B. Halliwell, J.M.C. Gutteridge, Free radicals in biology and medicine, 4th edition, Oxford University Press, Oxford, (2007).

[2] M. Ozyurek, B. Bektasoglu, K. Guclu, N. Gungor, R. Apak, J. Food Compos. Anal. 23 (2010) 689-698; https://dx.doi.org/10.1016/j.jfca.2010.02.013

[3] R. Apak, S. Gorinstein, V. Boehm, K. M. Schaich, M. Ozyurek, K. Guclu, Pure Appl. Chem. 85 (2013) 957-998; https://dx.doi.org/10.1351/PAC-REP-12-07-15

Progress

A Workshop titled Determining Antioxidants as Reactive Species Scavengers is being organized on 27-28 October 2014, in Istanbul, Turkey. The workshop will bring together experts from Australia, Greece, Portugal, Israel and Turkey and provide a forum among antioxidant researchers from all fields: food analytical chemistry, food technology, biochemistry and medicinal chemistry, and is expected to enable sharing the achievements of the IUPAC project with other participants. See details at https://antioxidantworkshop2014.istanbul.edu.tr/

Sep 2017 update – a final technical report provisionally titled “Methods to evaluate the scavenging activity of antioxidants toward reactive oxygen and nitrogen species (ROS/RNS)” is in preparation and will be submitted to Pure and Applied Chemistry before the end of 2017. In this report, the task group focuses on the applications and impact of existing assays on potentiating future research and innovations to evolve better methods enabling more comprehensive study of different aspects of antioxidants, and to provide a vocabulary of terms related to antioxidants and scavengers for ROS/RNS. The main methods comprise the scavenging activity measurement of hydroxyl radical (HO•), superoxide anion radical (O2●–), hydrogen peroxide (H2O2), hypochlorous acid (HOCl), peroxynitrite (ONOO-), and peroxyl radical (ROO•). In spite of the diversity of methods, there is currently a great need to evaluate the scavenging activity of antioxidant compounds in vivo and in vitro. In addition, there are incorrect methods frequently used, such as non-selective UV measurement of H2O2 scavenging, producing negative errors due to incomplete reaction of peroxide with flavonoids in the absence of transition metal ion catalysts. The task group also discusses the basic mechanisms of spectroscopic and electrochemical nanosensors for measuring ROS/RNS scavenging activity of antioxidants. This project aids the identification of reactive species and quantification of scavenging extents of antioxidants through various assays, make the results comparable and more understandable, and bring a more rational basis to the evaluation of these assays and provides a critical evaluation of existing ROS/RNS scavenging assays to analytical, food chemical, biomedical/clinical communities by emphasizing the need of developing more refined, rapid, simple and low-cost assays, and thus opening the market for a wide range of analytical instruments, including reagent kits and sensors.

Page last updated: 1 Sep 2017