Project Details Methods of measurement and evaluation of natural antioxidant capacity/activity

Project No.:
Start Date:
01 February 2009
End Date:
01 May 2013


To bring in terms of definitions or definition-like characterization and classification the chemical and biochemical methods of antioxidant assays as well as related antioxidants chemistry and to provide analytical, food chemical, biomedical/clinical and environmental communities with critical evaluation on this topic.


The chemical diversity of natural antioxidants makes it difficult to separate, detect and quantify individual antioxidants from the complex matrix. Moreover, the total antioxidant power is often more meaningful to evaluate health beneficial effects because of the cooperative action of individual antioxidant species. Currently there is no single antioxidant assay for food labeling because of the lack of standard quantification methods.

Antioxidant assays may be broadly classified as the electron transfer (ET) – and hydrogen atom transfer (HAT)-based assays. The results obtained are usually hardly comparable because of the different mechanisms, redox potentials, pH- and solvent- dependencies, etc., in various assays.
This project will aid to identify and quantify of properties and mutual effects of antioxidants, to bring a more rational basis to the classification of antioxidant and antioxidant assays and to make the results more comparable and understandable.
The task group members are experienced in various methods of antioxidants assay. The chairperson has developed a novel CUPRAC (cupric reducing antioxidant capacity) method [1] which has been successfully applied to antioxidants assay in food plants, human serum, and to hydroxyl radical scavengers [2].

[1] R. Apak, K. G��l�, M. �zy�rek, S. E. Karademir, J. Agric. Food Chem. 52 (2004) 7970-7981. doi:10.1021/jf048741x
[2] M. �zy�rek, B. Bekta?o?lu, K. G��l�, R. Apak, Anal. Chim. Acta, 616 (2008) 196-206. doi:10.1016/j.aca.2008.04.033���


> project announcement published in Chem. Int. July-Aug 2009

> As part of this project, the task group is coordinating a workshop on Antioxidant Measurement Assay Methods, on�21 April 2010;

see website for more details: or link to conference calendar

> May 2013 – Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC Technical Report) published in Pure Appl. Chem., 2013, Vol. 85, No. 5, pp. 957-998; (first published online 26 Feb 2013)

project completed

Last updated 7 June 2013